Abstract: PD48-12
Sources of Funding: Non available _x000D_

Introduction

Cystoscopy remains the main stay of surveillance employed for the follow up of Bladder cancer patients. The present study aims to provide a new detection method which is completely non-invasive, does not require additional surgery, and which is more accurate than visual inspection techniques, using a new photodynamic diagnostic procedure to quantify certain cancer specific biomarker called Porphyrin, which selectively binds on to the bladder cancer tissues.

Methods

Twenty bladder cancer patients and twenty healthy controls (all confirmed by cystoscopy) were invited to participate in the current clinical trial (NCT02101931), where 5 mg of 5-Aminolevulinic Acid (ALA) per kilogram body weight were administered for each subject. ALA gets metabolized into certain types of porphyrins which selectively bind on to the tumor tissues (for a longer time than the normal tissues which is two hours). At two hour intervals after administration, blood and urine sample were collected from the patients and were optically analyzed to measure a concentration of porphyrin therein. Optical analysis is performed by laser-induced fluorescence spectroscopy using a blue diode laser with a power rating about 100 mW and a wavelength of 405 nm.

Results

The laser-induced fluorescence spectroscopy results for the detected porphyrin in the blood and urine samples shows that approximately 90% of porphyrin were eliminated through urine for a healthy person after four hours. However, in cancer patients’ blood and urine samples, the prophyrin were retained up to eight hours, which indicates that healthy controls shows low signal intensity than bladder cancer patients in both urine (Fig 1) and blood (Fig 2) samples.

Conclusions

The present technique offers a viable, easy and reliable tool for diagnosis and continual monitoring of disease regression through blood and urine, based on optical analysis. Further studies on a large scale are warranted for conformation of these results.

Funding

Non available _x000D_