Abstract: PD25-04
Sources of Funding: Coloplast Corp.

Introduction

Previous studies have used traditional culture methods to identify microbial biofilm composition present at removal and replacement of penile prostheses. Molecular analysis (PathoGenius Laboratories, Lubbock, TX) PCR amplifies extracted 16S ribosomal DNA, which is then sequenced and compared to known bacterial and fungal taxonomies to identify isolates and microbial susceptibilities. The purpose of this study was to identify biofilm compositions and antimicrobial sensitivities/resistances at penile prosthesis removal/replacement using 16S ribosomal DNA testing.

Methods

Level 1 testing rapidly analyzed samples for most commonly found microbes in wound or ear, nose, and throat samples. Each sample composition was identified by quantitative PCR analysis with a specified panel of pathogens. Level 2 testing universally assessed for relative quantities of bacteria and fungi. DNA sequencing methods were used to identify the pathogens’ genetic signatures and the estimated percentages of organisms present in each specimen. Biofilm pathogens were evaluated against 34 unique antimicrobial agents.

Results

Intraoperative penile prosthesis capsule specimens were submitted for analysis at the time of revision surgery. Quantitative PCR analysis of common pathogens resulted in 14 specimens with low bacterial loads and 4 specimens with medium bacterial loads. 16S ribosomal DNA testing produced positive results in 5 of 18 specimens. One of these specimens had 2 separate fungi, while the other four positive specimens had 9, 13, 14, and 8 separate bacteria species respectively. Some of the bacteria identified during 16S ribosomal DNA testing were known prosthetic infectious pathogens. All isolates had sensitivities/resistances to 34 unique antimicrobial agents, enabling the physician to accurately tailor treatments for each patient.

Conclusions

16S ribosomal DNA molecular testing has proven to be beneficial in its thorough analysis of biofilm composition and antimicrobial sensitivities/resistances on inflatable penile prostheses at the time of revision surgery. Future identification of biofilm and antimicrobial sensitivities/resistances may be improved with 16S ribosomal DNA molecular testing.

Funding

Coloplast Corp.