Login to Access Video or Poster Abstract: MP99-17
Sources of Funding: This work was supported by NIH grant CA156700 and George Whipple Professorship Endowment, and Taiwan Ministry of Health and Welfare Clinical Trial and Research Center of Excellence (DOH102-TD-B-111-004), and National Natural Science Foundation of China ?81672526?;

Introduction

Circular RNAs (circRNAs) as a form of non-coding RNA could affect the formation and development of tumors. The expression of the androgen receptor splicing variant ARv7 has been demonstrated to play a key role for the development enzalutamide (Enz) resistance in castration-resistant prostate cancer (CRPC).

Methods

Western blot?invasion assay and chip assay were used.

Results

circRNA17 (hsa_circ_001305) has a lower expression in C4-2 Enz-resistant cells compared to that in C4-2 parental cells. Knocking down circRNA17 in C4-2 parental cells increased the expression of ARv7 that resulted in decreased sensitivity to Enz and increased cell invasion.

Conclusions

circRNA17 can alter the Enz sensitivity and cell invasion in CRPC cells via modulating the miR-181c-5p-ARv7 signaling and targeting this newly identified signaling may help us to develop a better therapy to further suppress the CRPC.

Funding

This work was supported by NIH grant CA156700 and George Whipple Professorship Endowment, and Taiwan Ministry of Health and Welfare Clinical Trial and Research Center of Excellence (DOH102-TD-B-111-004), and National Natural Science Foundation of China ?81672526?;