Introduction

Diabetes type-2 accounts for almost 90% of diabetes cases worldwide. Diabetes among other complications induces bladder dysfunction. In the current study we aimed to create a short-period diabetes type-2 model in order to investigate oxidative stress-related alterations in the bladder in the initiation of the disease. Additionally antioxidant treatment with resveratrol or taurine was provided in order to examine whether it is possible to prevent these alterations in the very beginning of the disease.

Methods

Diabetes was induced in 8-week-old male Wistar rats with a single dose of streptozotocin (40mg/kg) intraperitoneally (i.p.). The next day they were randomly separated into 3 groups and 14 days feeding with a high fat diet followed. One group received no treatment (DM group), another group received orally resveratrol (10mg/Kg; Resv group) and the third one received taurine i.p. (1g/Kg; Tau group). Age matched control animals were used and were fed with normal diet (Control group). Two weeks later animals were sacrificed and bladder were processed for histological evaluation, measurements of malondialdehyde (MDA) and immunohistochemistry (IHC) for oxidative stress markers.

Results

At the end of 2 weeks all diabetic groups had significantly lower body weight compared to the Control group. DM group demonstrated significantly higher ratio of bladder weight to body weight compared to the Control (Figure 1). Histological evaluation demonstrated mild damage of the bladder tissue in the DM group such as abruption of the mucosa from the muscularis as well as edema in the transitional epithelium. All these alterations were not observed in the treatment groups. MDA levels in the bladder were significantly larger in the DM group compared with Control, Resv or Tau group (Figure 1). Fourteen days of diabetes without treatment in the DM group induced moderate to strong intensity and positivity for oxidative stress marker MDA, 4-Hydroxynonenal and DNA oxidative stress marker 8-deoxyguanosine compared with the other three groups.

Conclusions

The prompt diagnosis of diabetes can be crucial for the progression of the disease. Specifically in the bladder, it appears that both mild damage in structural level as well as oxidative damage in molecular level can be prevented by resveratrol or taurine treatment.

Funding

None