Introduction

Surgical techniques that apply temporary kidney ischemia are currently widely used. Post ischemia-reperfusion (IR) is essential for ischemic tissue survival.The term ischemic preconditioning (IPC) is defined as the short ischemic period followed by a brief period of reperfusion that typically precede a longer period of definitive ischemia. _x000D_ This study aimed to evaluate the effect of IPC on kidney subjected to IR by using dosing markers of oxidative damage in the kidney tissue._x000D_ _x000D_

Methods

Seventeen Wistar male rats were randomized into group 1 (n=6), animals subjected to IR; group 2 (n=6), animals subjected to preconditioning before IR; and group 3 (n=5), subjected to sham._x000D_ We performed the following procedure for each group:_x000D_ a) in the IR group, the animals were subjected to 45-min left renal ischemia and 60-min reperfusion._x000D_ b) in the IPC group, we conducted three periods of 3 min (each time) of renal blood flow interruption with the use of a bulldog vascular clamp on the left renal pedicle, interposed with 5 min of reperfusion. Shortly thereafter, the left kidney was subjected to 45-min ischemia and, later, to 60-min reperfusion._x000D_ c) In the sham group, we conducted only left renal pedicle manipulation and killed the animals after 105 min. (45+60)._x000D_ _x000D_ Were evaluated markers of stress oxidative. _x000D_ _x000D_

Results

_x000D_ The total antioxidant capacity and total content of the sulfhydryl groups showed similar values in the sham group (24.44±1.90 and 1.41±0.12, respectively) compared with the group IR and PIC group (18.48±1.77 and 1.20±0.08, respectively). These values were higher when compared with those in the IR group (9.22±2.16 and 1.01±0.07)._x000D_ The enzymes that comprise the endogenous antioxidant system (glutathione peroxidase and glutathione reductase), the activities were reduced in the IR and IPC groups compared with the sham group. IPC group showed higher values compared with the ischemic reperfusion group._x000D_ The malondialdehyde increased in the IR group compared with the animal of sham group and IPC group. _x000D_ The carbonyl protein and caspase-3 contents were higher in the IR group than in the sham group. The PIC group demonstrated similar averages for both protein carbonyls and caspase 3 as those in the IR group._x000D_

Conclusions

IPC contributed to reducing cell damage by kidney IR in animals subjected to brief periods of vascular pedicle clamping._x000D_

Funding

none