Login to Access Video or Poster Abstract: MP73-16
Sources of Funding: None

Introduction

The ischaemia and subsequent reperfusion (IR) which occurs in partial nephrectomy used in the treatment of renal tumours causes loss of parenchyma in the damaged kidney. The aim of this study was to investigate the protective effect of Esomeprazole, which is used in the suppression of gastric acid, on a rat model of IR.

Methods

The 21 rats were separated into 3 groups of 7. In Group 1 (sham), only laparotomy was applied and no renal ischaemia was formed. In Group 2 (control), ischaemia was formed by applying a cross-clamp to the left renal artery. In Group 3 (PPI), Esomeprazole was administered 1 hour before the procedure, then ischaemia was formed. All the animals were sacrificed 24 hours after the procedure. Biochemical analyses were applied for evaluation of oxidant and antioxidant agents in the blood and left kidney of each subject (oxidative markers: malondialdehyde, myeloperoxidase; antioxidant marker: superoxidedismutase). In the histological examination of the kidney tissues stained with haematoxylin-eosin, the TUNEL method was applied in the evaluation of apoptosis.

Results

No statistically significant biochemical difference was determined in the blood and tissue samples. In the histological and apoptosis evaluations, a statistically significant difference was determined between the sham, control and PPI groups. The median (interquartile range) values of the TUNEL positive cells were counted as 1.50 (4) in the Sham group, 11.50 (12) in the Control group and 6.00 (9) in the PPI group (p<0.001).

Conclusions

A protective effect of Esomeprazole was confirmed in renal ischaemia-reperfusion damage created in an experimental rat model.None

Funding

None