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Sources of Funding: Scientific and Technical Research Council of Turkey (TUBITAK), Grant No: 114S738._x000D_

Introduction

Peyronie's disease (PD) is characterized by the formation of fibrous plaques in the tunica albuginea (TA). It causes penile curvature, erectile dysfunction and pain during erection. Our aim is to evaluate whether or not Mitomycin-C (MMC) has an antifibrotic effect on (Transforming Growth Factor-beta (TGF-?) induced PD in a rat model.

Methods

Eighteen 12-week-old male Sprague-Dawley rats were divided into 3 groups: Group 1= TGF-?1 (n:7), Group 2= TGF-?1+MMC (n:7), and Group 3= Sham group (0.25 ml bovine serum albumin injected) (n:4). All groups were sacrificed on the 6th week of the procedure, and their penises were excised. All penis specimens were evaluated semiquantitatively and quantitatively with histochemical, immunohistochemistry and image analysis.

Results

Both group 1 and group 2 had significantly higher fibrosis scores and lower elastic fibers in both outer surface of TA and subsinusoidal area compared with sham group (Group 3). When compared group 1, the amount of collagen were significantly decreased in group 2. Intracavernosal MMC injection (group 2) ended up lower elastic fibers when compared with group 1(Table 1-Figure 1)._x000D_ According to the quantitative analyses, when compared with group 1 and group 3, lower dorsal, ventral and trabecular thickening values were seen in group 2. These parameters were only statistically significant when compared with group 1, suggesting the antifibrotic effect of TGF-?1 induced fibrosis (Table 2-Figure 1)._x000D_ Both group 1 and group 2 showed lower decorin staining levels in subsinusoidal areas of tunica albuginea (SATA) and subsinusoidal areas of trabecular wall (SATW) when compared with group 3. The statistically significant difference was only detected between group 1 and group 3. _x000D_

Conclusions

The fact that amount of collagen bundles decreased and elastic fibers increased in MMC group (group 2) compared TGF-?1 group (group 1) was demonstrated by histopathological (semi-quantitative) and image analysis program (quantitative). These results were statistically significant. Our study demonstrates the antifibrotic effects of MMC on Peyronie Disease. Further clinical studies are assured to make some inferences regarding its clinical use.

Funding

Scientific and Technical Research Council of Turkey (TUBITAK), Grant No: 114S738._x000D_