Login to Access Video or Poster Abstract: MP48-07
Sources of Funding: Independent research program of Tsinghua University

Introduction

To investigate the practicability of bladder mucosa reconstruction with autologous peritoneal free graft for treatment of nonmuscle invasive bladder cancer in animal models.

Methods

24 New Zealand white rabbits (weighing 2.0-3.0 kg) were randomly divided into 2 groups. After avulsing all of the bladder mucosa, the autologous peritoneal free grafts from posterior abdominal wall were used to replace rabbit bladder mucosa in the experimental group (n=12), and the incision was directly sutured after incising the bladder in control group (n=12). The survival condition of animals was observed after operation. At 15 days, 1, 3, and 6 months after operation, the blood routine, urine routine,renal function, and electrolyte were tested by collecting the blood and urine samples. At 1, 3, and 6 months after operation, maximum bladder capacity, bladder leak point pressure, and bladder compliance were measured through urodynamic studies. Then gross observation was performed for the bladder, and the specimens of the bladder were harvested for HE staining ,masson staining and scanning electron microscope (SEM). For immunohistochemistry, tissue sections were stained with antibodies to pancytokerotins AE1/AE3, UPK3 and calretini.

Results

All the animals survived during the experiment. The tests of urine routine,renal function and electrolyte suggested no significant difference between 2 groups (P>0.05). The maximum bladder capacity and compliance in experimental group were significantly lower than those in control group at 3 and 6 months after operation (P<0.05), but no significant difference in bladder leak point pressure at each time point between 2 groups (P > 0.05). In experimental group at 1 months after operation, no peritoneum mesothelial cells were observed?and histological examination showed the constitutions of the reconstructed bladder mucosa were similar to the normal appearance?Neovascularization was evident beneath the mucosa and some chronic inflammatory cells and fibroblasts were present in the subepithelial layer.At 3 and 6 months after operation, the bladder mucosa was uniformly lined with 3-5 lays transitional epithelium?Immunohistological analysis of the epithelial cells demonstrated negative expression of calretini and positive expression of both CK AE1/AE3 and UPK3.

Conclusions

The autologous free peritoneal grafts could be successfully used as the bladder mucosa reconstruction.

Funding

Independent research program of Tsinghua University