Login to Access Video or Poster Abstract: MP45-04
Sources of Funding: none

Introduction

Cisplatin treatment leads to cytotoxic events and generates oxidative stress, which has deleterious effects on the function of several organ systems, including the corpus cavernosum. The present study was designed to investigate the putative beneficial effect of quercetin (QT) against cisplatin-induced corpus cavernosum damage.

Methods

Twenty-eight adult male Sprague Dawley rats were included in the study. Six-teen rats were administered intraperitoneally (i.p.) single dose cisplatin 7?mg / kg and divided in to 2 groups: The first group (n=8) received saline i.p., whereas the second group (n=8) fed orally with 20?mg / kg QT, respectively, for 21 days. The remaining 12 rats served as the control group after i.p. saline, where 6 treated with QT. _x000D_ After decapitation, corpus cavernosum strips were placed in organ bath and isometric contractions to phenylephrine and relaxations to carbachol (10-8 to 10-4 M) were recorded. In order to examine oxidative tissue injury, malondialdehyde (MDA), 8-hydroxydeoxyguanosin (8-OHdG) and glutathione (GSH) levels and superoxide dismutase (SOD) and caspase-3 activities and caspase-3 protein expression in corpus cavernosum tissues were measured along with histologic evaluations. _x000D_

Results

In the cisplatin-treated corpus cavernosum, the contractile responses were lower than those of the control group and were reversed by treatment with QT (figure 1). On the other hand, increase in MDA and 8-OHdG levels, and caspase-3 protein expression and caspase 3 activities of tissues in the cisplatin group were significantly reversed by QT treatment (figure 2 and 3). Furthermore, treatment with QT also prevented the depletion of tissue GSH levels and SOD activity seen in the cisplatin group (figure 4). Histologic evaluations also supported the beneficial effects of QT treatment on corpus cavernosum.

Conclusions

According to the results, QT exerts beneficial effects against cisplatin-induced damage on corpus cavernosum through its anti-inflammatory and antioxidant effects.

Funding

none