Login to Access Video or Poster Abstract: MP44-07
Sources of Funding: None

Introduction

Circular RNAs with a special form of non-coding RNAs play an important role in the development of human diseases, but few are known in various cancers. In the present study, we tried to explore the clinical application of circRNAs in bladder cancer.

Methods

Sanger sequencing, divergent primer amplification and RNAse R digestion were used to identify existence of a novel circRNA (circLPAR1,hsa_circ_0087960) from deep sequencing of RNA for bladder cancer. It was detected in 146 cancer tissues and 20 cases paracancer tissues to compare the different expression by qRT-PCR. A univariate and multivariate Cox regression was carried out to explore the correlation between circLPAR1 expression level and the overall survival(OS) of patients. We further investigated the possible network for circRNA-miRNA-mRNA by bioinformatics analysis.

Results

CircLPAR1 was composed of two exons with the size of 226bp. It was confirmed the objective existence and the circular structure, and first found to be significantly downregulated in bladder cancer tissues compared with paired paracancer tissues (P<0.001), which was verified by an external validation of other 60 pairs specimens(P=0.0001).On the univariate and multivariate analysis for 146 patients, a low circRNA expression level (2^{-?CT?0.0023) was significantly associated with poor OS compared to a high circRNA expression level (2-?CT?0.0023), and the mean OS was 64.9 months and 87.6 months, respectively (P<0.001). Four miRNA(hsa-miR-762, hsa-miR-323b-5p, hsa-miR-1183, hsa-miR-920) might be correlated with circLPAR1 and circRNA-miRNA-mRNA interaction network was drawn._x000D_}

Conclusions

Our findings suggested that circLPAR1(hsa_circ_0087960) might be a novel potential biomarker for the prognosis of bladder cancer and low expression of circLPAR1 indicated poor survival.

Funding

None