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In vivo fluorescence imaging of an orthotopic rat bladder tumor model indicates differential uptake of intravesically instilled near-infrared labeled 2-deoxyglucose analog by neoplastic urinary bladder tissues

Login to Access Video or Poster Abstract: MP44-01
Sources of Funding: University of Oklahoma

Introduction

Bladder cancer is one of the most expensive cancers to manage due to frequent recurrences requiring life-long surveillance and treatment. Deoxyglucose radionucleotides have been shown to detect metastatic bladder cancer. This pilot study has explored differential uptake of intravesically administered IRDye800CW-DG, a near-infrared labeled 2-deoxy-d-glucose probe targeting glucose metabolism pathway, to detect surface cancer in an orthotopic rodent bladder tumor model.

Methods

Twenty-five female Fischer rats were randomly grouped to four conditions: control (n=3), control and intravesically instilled with IRDye800CW-DG (n=6), treated with GFP-labeled AY-27 rat bladder urothelial cell carcinoma cells and washed with saline (n=5), and treated with AY-27 and intravesically instilled with IRDye800CW-DG (n=11). Near-infrared fluorescence was measured from the opened bladder wall of anesthetized rat at an excitation wavelength of 750nm and an emission wavelength of 776nm, by using an in-house fluorescence imaging system.

Results

There is no statistically significant difference of the peak fluorescence intensity among the control bladders (n=3), the control bladders instilled with IRDye800CW-DG (n=6), and the GFP-labeled AY-27 treated bladders washed by saline (n=5). When compared to that of the control bladders instilled with IRDye800CW-DG (n=6), the fluorescence intensity of GFP-labeled AY-27 treated bladders instilled with IRDye800CW-DG and with histology confirmed neoplastic bladder tissue (n=11) was remarkably stronger (3.34 folds of the former) that was also statistically significant (p<0.0001).

Conclusions

The differential uptake of IRDye800CW-DG by the neoplastic urinary bladder tissues suggests the potential for cystoscopy-adaptation to enhance diagnosis and guiding surgical management of flat urinary bladder cancer.

Funding

University of Oklahoma

Authors
Joel Slaton
Carole Davis
Robert Hurst
Daqing Piao
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