Login to Access Video or Poster Abstract: MP41-04
Sources of Funding: Korea Healthcare Technology R&D project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (HI13C1398)

Introduction

To examined the therapeutic effects of mesenchymal stem cell (CHA1) on damaged bladder tissue in a chemically induced chronic interstitial cystitis (IC) rat model.

Methods

Female 10-week-old Sprague-Dawley rats were used for induction of chronic IC model._x000D_ Chronic IC model was induced with single intravesical instillation of protamine sulfate (0.5ml of PS, 30mg/ml) and lipopolysaccharide (0.5ml of LPS, 2.25mg/ml) for 1 month. Rats were divided into three groups (N=5~7): non treated control group(Control), sham operation group(Sham), chronic IC receiving a single bladder submucosal injection of phosphate-buffered saline (20 mL, PBS) or chronic IC treated with CHA1 (5×105 cells/20 μL, IV or BL(submucosa injection)). Four weeks after treatment, voiding spot was obtained using 6hr metabolic cage and analyzed with Image J program. The bladder was harvested for histologic examinations and toluidine blue staining for mast cell. All animal experiments were approved by the Institutional Animal Care and Use Committee at our institution._x000D_

Results

Rats in the IV and BL group showed increased voiding frequency and decreased the spot size compared with control sham group (p<0.05). Significant improvement of voiding spot pattern in size was observed in the CHA1 treated IV and BL groups compared with PBS group (Figure 1). Hematoxylin/eosin staining demonstrated that loss of urothelial integrity in PBS group was restored in IV and BL group. Neutrophil infiltration was also decreased in IV and BL group compared with PBS group. Histological examination revealed a significant decrease in the total number of infiltrated mast cells in IV and BL rats compared with the PBS rats (p<0.05), (Figure 2).

Conclusions

This is the first study to investigate the efficacy of CHA1 for chronic IC model. Submucosal injection of CHA1 in chronic IC showed improvement of voiding pattern and histological restoration and this suggest that CHA1 might have a therapeutic potential for IC. Further studies are required to evaluate the mechanism of CHA1 treatment.

Funding

Korea Healthcare Technology R&D project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (HI13C1398)