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Metformin reduce the renal stone formation in high oxalic acid rats by inhibiting the activation of NLRP3 pathway

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Sources of Funding: none

Introduction

metformin is a common oral drug which has been reported to treat diabetes and other diseases. The aim of this study was to explore the role and mechanism of metformin in reducing the incidence of kidney stone.

Methods

SD rats were randomly divided into 4 groups: group A (normal rats group, n=8), normal rats; group B (normal rats were given intragastric administration of metformin group, n=8); group C (hyperoxaluria rats, n=8 rats) 0.5% glycol and 1% ammonium chloride in drinking water; group D (hyperoxaluria rats were given intragastric administration of metformin group, n=8) same processing as group C, while giving the daily intragastric administration of metformin. The 24 hours’ urine of rat’s were collected before nephrectomy. Tissue sections were stained with Von Kossa to observe the crystallization, expression of OPN, CD44, NLRP3, IL-1, caspase-1 were by real-time quantitative PCR and Western Blotting.

Results

The urinary oxalate in C and D group was significantly higher than that in A group and B group (P<0.05). Von Kossa staining showed that crystal deposition of A, B, C, D group were 0, 0, 81.25% and 93.75%, respectively. The crystal density of group C was significantly higher than that in group D (P<0.05). OPN, CD44, IL-1, caspase-1 and NLRP3’s mRNA and protein expression of C, D group was significantly higher than the other two groups (P<0.05), the C group was significantly higher than the D group (P<0.05)._x000D_

Conclusions

Metformin inhibits the production of NLRP3 in rats with high oxalate, which can reduce the activation of the inflammatory bodies and thus reduce the production of kidney stones._x000D_ Key words: kidney calculi_x000D_

Funding

none

Authors
Hongyang Jiang
Tao Wang
Zhuo Liu
Jihong Liu
Shaogang Wang
Zhangqun Ye
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